Immunization and boosts of 5 Balb/c mice with standard immunization protocol.

ELISA evaluation of titer prior to selection for fusion.

Approximately 3-4 mg protein immunogen or 2 mg conjugated peptide immunogen and 0.5 mg free peptide are required.

Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from mouse with the best titer and myeloma cells.

Supernatants from the growing hybridoma wells will be screened by ELISA. If needed, 10 clones will be sent to customer's lab for evaluation

2 of these 10 clones selected by the customer will be subcloned by limiting dilution, isotyped, and expanded for cryopreservation.

$4,500

Conjugated peptides or recombinant proteins are used to immunize rabbits.

Ten-week protocol to produce antibodies in 2 rabbits. Pre-immune and three immune bleeds (100-120 ml antiserum).

$2250

10-13
Weeks