Our Custom Monoclonal Antibody Service Gives You:
-
Immunization of 5 Balb/c mice.
Fusion of spleen cells with SP2/0 myeloma cells.
ELISA screening for selection of antibody producers.
Subcloning and expansion of selected antibody producers.
Breakdown of Standard Monoclonal Service by Phase |
|
Phase I 6 Weeks |
Immunization and boosts of 5 Balb/c mice with standard immunization protocol. ELISA evaluation of titer prior to selection for fusion. Approximately 3-4 mg protein immunogen or 2 mg conjugated peptide immunogen and 0.5 mg free peptide are required. |
Phase II 4-6 Weeks | Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from mouse with the best titer and myeloma cells. Supernatants from the growing hybridoma wells will be screened by ELISA. If needed, 10 clones will be sent to customer's lab for evaluation |
Phase III 4-8 Weeks | 2 of these 10 clones selected by the customer will be subcloned by limiting dilution, isotyped, and expanded for cryopreservation. |
Total Cost |
$4,500 |
Our Custom Polyclonal Antibody Service Gives You:
Service | Description | Price | Time |
|
1 | Immunization and anti-sera production |
Conjugated peptides or recombinant proteins are used to immunize rabbits. Ten-week protocol to produce antibodies in 2 rabbits. Pre-immune and three immune bleeds (100-120 ml antiserum). | $1500 | 7-10 weeks |
2 |
Test |
ELISA test until the titer reaches 1:4,000. | Included | 1 day |
3 |
Purification |
Protein G purification Antibodies are eluted using both pH11 and pH2.5 buffers and neutralized immediately followed by dialysis in PBS. | $750 | 1 week |
Total |
$2250 | 10-13 Weeks |
For more information, please contact:
- Bassam Damaj, Ph.D.
COO, CSO
6330 Nancy Ridge Drive, Suite 103
San Diego, CA 92121 USA
Tel: 858.663-1159; Fax: 858.587-2131
E-mail: bdamaj@bio-quant.com
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